Garcinia kola is cultivated for its economic importance. The plant is mainly used as a medicinal herb in West Africa. Although it is bitter the plant is used as a snack and a stimulant due to the high content of caffeine in the seeds of the plant. The current study was done to investigate the antibacterial activity of the plant seeds and to analyse the presence of important pharmaceutical compounds. From the study the plant seeds were found to contain tannins, saponins, flavonoids, terpenoids, glycosides and alkaloids but phenols and steroids were found to be absent in the plant seeds. Among all the organisms tested with the ethanol extract of G. kola, only B. cereus with an inhibition zone of 10.17±0.477 and E. coli with an inhibition zone of 12.83±0.833 were inhibited. All other organisms were not inhibited. The penicillin control showed large zones of inhibition and DMSO did not show any zones of inhibition. The data obtained in this research is a scientific justification of the plants traditional use in the treatment of various stomach problems. From this research it is worthy to recommend the plant seeds for the treatment of diarrhea caused by E.coli and all the ailments caused by B. cereus, however further research needs to be done to isolate the pharmaceutical compounds, investigate their mode of action and the effect of the same in the in vivo environment.