The aim of the current work is to investigate the antibacterial activity of infused Cola nitida seeds. Antibacterial screening was done using agar well diffusion method against Bacillus cereus, Serratia marcescens, Staphyloccus epidermidis, Proteus vulgaris and Salmonella typhi. The extract inhibited the growth of Bacillus cereus with a zone of inhibition of 14.33±0.882, Serratia marcescens 26.67±1.202 and Staphylococcus epidermidis 12.33±0.577. The penicillin control showed large zones of inhibition and DMSO did not show any zones of inhibition. Analysis of variance (ANOVA) performed on the data showed that there were significant differences in the zone of inhibition formed by the extract and antibiotic control (p<0.0001). A Tukey s multiple comparison tests also revealed several differences in the zones of inhibition of the extract between organisms and between all the organisms and their controls. Few pairwise comparisons showed that the zones did not differ significantly between organisms. The present study has proved that it is possible to control the growth of some pathogenic microorganisms using the infused extract of Cola nitida. The infused extracts from the plant can therefore be considered by pharmaceutical industries in production of cheap, affordable and available drugs for the cure of infections caused by these organisms in situations where expensive conventional drugs are unavailable.