PRELIMINARY PHYTOCHEMICAL AND ANTIBACTERIAL SCREENING OF FRESH TETRADENIA RIPARIA LEAVES WATER EXTRACT AGAINST SELECTED PATHOGENIC MICROORGANISMS
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Date
2014-07-07Author
Ngule, Mutuku Chrispus
Ndiku, Mueni H.
Francis, Ramesh
Ngule, Makau Elijah
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The use of plants as a source of medicine is as old as man himself. Plants have been used since time
immemorial to treat against various diseases affecting human beings all over the world. Before the invention of
synthetic drugs, traditional medicine dominated the world. The study was conducted to analyze the antibacterial
activity phytoconstituents of Tetradenia riparia plant. The samples were extracted using water solvent. The
phytoconstituents study was done using standard procedures. The bioassay was done using well diffusion method.
From the study, Tetradenia riparia was found to contain tannins, saponins, flavonoids, cardiac glycosides, phenols,
alkaloids and steroidal rings, but terpenoids, steroids and steroidal nucleus were found to be absent in the plant
extract. The fresh leaves water extract of the plant Tetradenia riparia inhibited the growth of all the
microorganisms used. The zones of inhibition were high in Salmonella typhi (21.00±0.000), followed by
Staphylococcus epidermidis, Proteus vulgaris and Bacillus cereus with inhibition zones of 18.67±0.577, 18.00±0.333
and 18.00±0.577 respectively. Serratia marcescens and Serratia liquefaciens had inhibition zones of 13.00±0.333 and
13.33±0.577 respectively. Escherichia coli had the least inhibition zone of 9.67±0.577. Penicillin which was used as the
positive control inhibited the growth of all the microorganisms tested while DMSO (negative control) did not show
any inhibition zones against any of the microorganisms tested. The current study may be a partial scientific
justification of the plants use in the treatment against the infections caused by all the tested microorganisms.