Motivating employees is one of the most vital activities that managers have to perform, but it is also one of the most challenging. What motivates one individual does not necessarily motivate another. It is therefore a complex task to determine what motivates whom, and how to apply such motivational factors in practice. This study aimed at establishing the relationship between employees’ motivational levels and selected motivational factors in the banking sector in Kenya. The study adopted a cross sectional and descriptive – correlation research designs and targeted the bank employees from the various bank branches located within Nairobi County who were randomly sampled to give their responses on a set of items in a self - constructed questionnaire. The data was statistically analyzed through measures of central tendency and dispersion as well as the use of descriptive statistical tools mainly the mean and standard deviation and inferential tools mainly the analysis of variance (ANOVA) and Pearson correlation - moment. The study established that all the selected motivational factors have significant direct and moderate relationship with motivation level of employees and that that there is a significant difference between the motivation level of employees classified by age group, levels of education, gender, and duration of service in the Banking Industry. The study recommends that banks take into consideration the various elements of the motivational factors as well as the various demographic characteristics when formulating their motivational programs so as to ensure that the employees are well motivated.